Practice for Bio-organic Chemistry |
ASAHINA, Masashi |
| Elective Requisites 2 credits |
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【Biosciences･2nd semester】
16-1-0410-2639 |
| １． |
Objectives |
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Living organisms produce biologically active substances. For example, plants synthesize plant hormones which are involved in regulation of various growth and physiological phenomena in the life cycle. On the other hand, more than just a few microorganisms produce antibiotics, which we utilize as medicines. In this course, biological and chemical methodologies dealing with biologically active substances of plant and microorganism origin will be studied.
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| ２． |
Outline |
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1. Cultivation and isolation of actinomycetes derived from soil samples collected from various places in Japan
2. Purification of streptomycin from the fermentation broth of Streptomyces griseus using an ion exchange resin
3. Antibacterial activity against Bacillus subtilis by agar plate diffusion method
4. Extraction of gibberellins and brassinosteroids from plant materials and purification by column chromatography
5. Bioassay methods to determine biological activity of gibberellins and brassinosteroids
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