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(2019)

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Nucleic Acid Engineering

 MIYAMOTO. Koji
  Elective Requisites  2 credits
【Biosciences･1st semester】
19-1-0394-4946

１．
Outline
The purpose of this course is to learn the following.
　(1) DNA cloning: restriction enzyme, ligation of DNA, and plasmid
　(2) Analysis of the cloned DNA: DNA sequencing, PCR, etc.
　(3) Application of nucleic acid engineering technology
２．
Objectives
This course aims to understand the basic tools and techniques in nucleic acid engineering, and its applications.
３．
Grading Policy
Final grade will be calculated according to quizzes in each lecture (30%) and the final examination (70%). To pass, students must earn at least 60 points out of 100.
４．
Textbook and Reference
Reference book: "Kiso kara manabu" genetic engineering (in Japanese), T. Tamura, Yodosha, ISBN 978-4-7581-2083-8
５．
Requirements (Assignments)
Students have to prepare each lecture by reading the handout posted on LMS and summarizing the content of next lecture in a notebook. This preparation will take one hour.
There will be homework each week. Students should review the questions they did not understand in their homework. Quizzes in each lecture will contain similar questions. This review will take two hours.
６．
Note
Students should take "Molecular genetics" and "Genome Science". Please review the contents of "Molecular genetics" and "Genome Science". Handout will be posted on LMS. Part of quizzes will be performed using mobile-MARS.
７．
Schedule
１. What is genetic engineering?
２. Structure of DNA
３. Gene expression
４. Basic methods of handling nucleic acids
５. DNA amplification by PCR and gel electrophoresis
６. Cloning and vector
７. Plasmid, ristriction enzyme and DNA ligase
８. Introduction of recombinant gene
９. Recombinant protein expression and analysis
10. Generating DNA library and screening
11. Detection and sequencing of DNA
12. Gene expression analysis
13. Application of genetic engineering technology
14. Application of genetic engineering technology and its safety
15. Final examination and commentary
１．
Outline
The purpose of this course is to learn the following.
　(1) DNA cloning: restriction enzyme, ligation of DNA, and plasmid
　(2) Analysis of the cloned DNA: DNA sequencing, PCR, etc.
　(3) Application of nucleic acid engineering technology
２．
Objectives
This course aims to understand the basic tools and techniques in nucleic acid engineering, and its applications.
３．
Grading Policy
Final grade will be calculated according to quizzes in each lecture (30%) and the final examination (70%). To pass, students must earn at least 60 points out of 100.
４．
Textbook and Reference
Reference book: "Kiso kara manabu" genetic engineering (in Japanese), T. Tamura, Yodosha, ISBN 978-4-7581-2083-8
５．
Requirements (Assignments)
Students have to prepare each lecture by reading the handout posted on LMS and summarizing the content of next lecture in a notebook. This preparation will take one hour.
There will be homework each week. Students should review the questions they did not understand in their homework. Quizzes in each lecture will contain similar questions. This review will take two hours.
６．
Note
Students should take "Molecular genetics" and "Genome Science". Please review the contents of "Molecular genetics" and "Genome Science". Handout will be posted on LMS. Part of quizzes will be performed using mobile-MARS.
７．
Schedule
１. What is genetic engineering?
２. Structure of DNA
３. Gene expression
４. Basic methods of handling nucleic acids
５. DNA amplification by PCR and gel electrophoresis
６. Cloning and vector
７. Plasmid, ristriction enzyme and DNA ligase
８. Introduction of recombinant gene
９. Recombinant protein expression and analysis
10. Generating DNA library and screening
11. Detection and sequencing of DNA
12. Gene expression analysis
13. Application of genetic engineering technology
14. Application of genetic engineering technology and its safety
15. Final examination and commentary