Experiments in Microbiology |
SAKUDA, Shohei |
| Elective Requisites 2 credits |
|
【Biosciences･1st semester】
19-1-0409-4887 |
| １． |
Outline |
|
The purpose of this course is to learn the following techniques
(1) Ligation of the lacZ DNA with the plasmid
(2) Transformation of the recombinant plasmid
(3) Purification and identification of the recombinant plasmid
(4) Restriction enzyme mapping of the lacZ DNA
(5) Isolation of microbes from environments and their cultivation
(6) Classification of bacteria by Gram staining and PCR
Skills obtained by this class are related to DP2 and DP3.
|
| ２． |
Objectives |
|
The course aims at studying the basic techniques of the DNA cloning using the plasmid of Escherichia coli and cultivation of microbes.
|
| ３． |
Grading Policy |
|
Experiment report: 100% (A pass is a score of 60% or higher.)
|
| ４． |
Textbook and Reference |
|
Handouts are used.
|
| ５． |
Requirements (Assignments) |
|
Please read the handouts carefully before experiments.
|
| ６． |
Note |
|
N/A
|
| ７． |
Schedule |
|
１. Ligation of the lacZ DNA with the plasmid and transformation of the recombinant plasmid into E. coli.
|
２. Digestion of the recombinant plasmid DNA and separation of digested plasmid DNA by agarose gel electrophoresis
|
３. Preparation of the plasmid DNA by alkaline lysis method
|
４. Restriction enzyme mapping of the lacZ DNA
|
５. Preparation of media for cultivating bacteria and fungi
|
６. Inoculation of environmental microbes
|
７. Isolation of bacteria and observation of fungi
|
８. Classification of bacteria by Gram staining and PCR
|
９.
|
10.
|
11.
|
12.
|
13.
|
14.
|
15.
|
|
| １． |
Outline |
|
The purpose of this course is to learn the following techniques
(1) Ligation of the lacZ DNA with the plasmid
(2) Transformation of the recombinant plasmid
(3) Purification and identification of the recombinant plasmid
(4) Restriction enzyme mapping of the lacZ DNA
(5) Isolation of microbes from environments and their cultivation
(6) Classification of bacteria by Gram staining and PCR
Skills obtained by this class are related to DP2 and DP3.
|
| ２． |
Objectives |
|
The course aims at studying the basic techniques of the DNA cloning using the plasmid of Escherichia coli and cultivation of microbes.
|
| ３． |
Grading Policy |
|
Experiment report: 100% (A pass is a score of 60% or higher.)
|
| ４． |
Textbook and Reference |
|
Handouts are used.
|
| ５． |
Requirements (Assignments) |
|
Please read the handouts carefully before experiments.
|
| ６． |
Note |
|
N/A
|
| ７． |
Schedule |
|
１. Ligation of the lacZ DNA with the plasmid and transformation of the recombinant plasmid into E. coli.
|
２. Digestion of the recombinant plasmid DNA and separation of digested plasmid DNA by agarose gel electrophoresis
|
３. Preparation of the plasmid DNA by alkaline lysis method
|
４. Restriction enzyme mapping of the lacZ DNA
|
５. Preparation of media for cultivating bacteria and fungi
|
６. Inoculation of environmental microbes
|
７. Isolation of bacteria and observation of fungi
|
８. Classification of bacteria by Gram staining and PCR
|
９.
|
10.
|
11.
|
12.
|
13.
|
14.
|
15.
|
|
|