| Teachers | SAKUDA, Shohei | |
|---|---|---|
| Grade, Semester | Year 3 1st semest [Department of Biosciences, Faculty of Science and Engineering] | |
| Category | Special Subjects | |
| Elective, Credits | Elective 2credit | |
| Syllabus Number | 5D258 | |
In this course, principles and applications of solvent fractionation, chromatography, and instrumental analyses will be studied. Key words in this lecture are organic solvents, solvent fractionation, column chromatography, thin layer chromatography, gas chromatography, high performance liquid chromatography, ultraviolet-visible spectroscopy, infrared spectroscopy, 1H- and 13C-NMR spectroscopy, and mass spectrometry. Skills obtained by this class are related to DP2 and DP3.
The aim of this course is to deal with methods of extracting, purifying, and identifying naturally occurring substances such as biologically active substances and food ingredients.
Quiz every time and report: 40%
Finals: 60%
| Kind | Title | Author | Publisher |
|---|---|---|---|
| Textbook | Handouts are used. | ||
| References |
N/A
| 1 | Learn about solvents and solvent fractionation. |
| 2 | Learn about partition and adsorption chromatographies. |
| 3 | Learn about ion exchange, gel filtration, electrophoresis, and distillation. |
| 4 | Learn about high performance liquid chromatography (HPLC). |
| 5 | Learn about ultraviolet spectrum. |
| 6 | Learn about optical rotation and infrared spectrum. |
| 7 | Learn about basic nuclear magnetic resonance (NMR). |
| 8 | Learn about 1H-NMR. |
| 9 | Learn about 13C-NMR. |
| 10 | Learn about 2D-NMR and application of NMR. |
| 11 | Learn about analysis of NMR spectrum. |
| 12 | Learn about ionization methods in mass spectrometry (MS). |
| 13 | Learn about ion separation methods in MS, LC/MS, GC/MS, and analysis of mass spectrum. |
| 14 | Visit a laboratory to study real instruments of HPLC and MS as practice. |
| 15 | Finals and explanation. |
