Instrumental Analysis
TeachersSAKUDA, ShoheiStaffInfo
Grade, SemesterYear 3 1st semest [Department of Biosciences, Faculty of Science and Engineering]
CategorySpecial Subjects
Elective, CreditsElective 2credit
 Syllabus Number5D258

Course Description

In this course, principles and applications of solvent fractionation, chromatography, and instrumental analyses will be studied. Key words in this lecture are organic solvents, solvent fractionation, column chromatography, thin layer chromatography, gas chromatography, high performance liquid chromatography, ultraviolet-visible spectroscopy, infrared spectroscopy, 1H- and 13C-NMR spectroscopy, and mass spectrometry. Skills obtained by this class are related to DP2 and DP3.

Course Objectives

The aim of this course is to deal with methods of extracting, purifying, and identifying naturally occurring substances such as biologically active substances and food ingredients.

Grading Policy

Quiz every time and report: 40%
Finals: 60%

Textbook and Reference

TextbookHandouts are used.





1Learn about solvents and solvent fractionation.

2Learn about partition and adsorption chromatographies.

3Learn about ion exchange, gel filtration, electrophoresis, and distillation.

4Learn about high performance liquid chromatography (HPLC).

5Learn about ultraviolet spectrum.

6Learn about optical rotation and infrared spectrum.

7Learn about basic nuclear magnetic resonance (NMR).

8Learn about 1H-NMR.

9Learn about 13C-NMR.

10Learn about 2D-NMR and application of NMR.

11Learn about analysis of NMR spectrum.

12Learn about ionization methods in mass spectrometry (MS).

13Learn about ion separation methods in MS, LC/MS, GC/MS, and analysis of mass spectrum.

14Visit a laboratory to study real instruments of HPLC and MS as practice.

15Finals and explanation.