Nucleic Acid Engineering
TeachersMIYAMOTO. KojiStaffInfo
Grade, SemesterYear 2 2nd semest [Department of Biosciences, Faculty of Science and Engineering]
CategoryBasic Major Subjects
Elective, CreditsElective Requisites 2credit
 Syllabus Number5C225

Course Description

The purpose of this course is to learn the following.
 (1) DNA cloning: restriction enzyme, ligation of DNA, and plasmid
 (2) Analysis of the cloned DNA: DNA sequencing, PCR, etc.
 (3) Application of nucleic acid engineering technology

Course Objectives

This course aims to understand the basic tools and techniques in nucleic acid engineering, and its applications.

Grading Policy

Final grade will be calculated according to the midterm examination (40%) and the final examination (60%). To pass, students must earn at least 60 points out of 100.

Textbook and Reference

References"Kiso kara manabu" genetic engineering (in Japanese)T. TamuraYodosha, ISBN 978-4-7581-2083-8


Students have to prepare each lecture by reading the handout posted on LMS and summarizing the content of next lecture in a notebook. This preparation will take one hour.
There will be homework each week. Students should review the questions they did not understand in their homework. This review will take two hours.


Students should take "Molecular genetics". Please review the contents of "Molecular genetics". Handout will be posted on LMS.


1What is genetic engineering?
Replication of DNA
Gene expression
2Basic methods of handling nucleic acids
3DNA amplification by PCR and gel electrophoresis
4Cloning and vector
5Plasmid, restriction enzyme and DNA ligase
6Introduction of recombinant gene
7Recombinant protein expression and analysis
8Midterm examination and commentary
9Generating DNA library and screening
10Detection and sequencing of DNA
11Gene expression analysis
12Analysis of protein-DNA interaction
13Analysis of protein-protein interaction
14Application of genetic engineering technology and its safety
15Final examination and commentary