Practice for Bio-organic Chemistry
TeachersTAKAHASHI, SenjiStaffInfo
Grade, SemesterYear 2 2nd semest [Department of Biosciences, Faculty of Science and Engineering]
CategoryBasic Major Subjects
Elective, CreditsElective Requisites 2credit
 Syllabus Number5E233

Course Description

1. Cultivation and isolation of actinomycetes derived from soil samples collected from various places in Japan
2. Purification of streptomycin from the fermentation broth of Streptomyces griseus using an ion exchange resin
3. Antibacterial activity against Bacillus subtilis by agar plate diffusion method
4. Extraction of gibberellins and brassinosteroids from plant materials and purification by column chromatography
5. Bioassay methods to determine biological activity of gibberellins and brassinosteroids

Course Objectives

Living organisms produce biologically active substances. For example, plants synthesize plant hormones which are involved in regulation of various growth and physiological phenomena in the life cycle. On the other hand, more than just a few microorganisms produce antibiotics, which we utilize as medicines. In this course, biological and chemical methodologies dealing with biologically active substances of plant and microorganism origin will be studied.

Grading Policy

Grading will be decided based on reports. To pass, students must earn at least 60 points out of 100.

Textbook and Reference

KindTitleAuthorPublisher
TextbookA textbook will be distributed.
References

Requirements(Assignments)

Students have to prepare each experiment by reading the distributed textbook and summarizing the content of next experiment in a notebook. This preparation will take 90 min.
After the experiment, students have to write and summarize the results of the experiment in a notebook. This review will take 90 min.

Note

Schedule

1Cultivation and isolation of actinomycetes
Extraction of gibberellins and brassinosteroids
2Purification of gibberellins by column chromatography
3Purification of brassinosteroids by column chromatography
4Observation of actionomycetes
Quantification of streptomycin
5Purification of streptomycin using an ion exchange resin
Calculation of antibacterial activity by agar plate diffusion method
6Bioassay to determine biological activity of gibberellins and brassinosteroids
7Calculation of adsorption and extraction rates of streptomycin
8Calculation of biological activity of gibberellins and brassinosteroids
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